endtag nucleic acid labeling system (Vector Laboratories)
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Vector Laboratories
endtag nucleic acid labeling system
Endtag Nucleic Acid Labeling System, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/MB-9002/3'+EndTag+DNA+End+Labeling+System/bio_rxiv__2025__08__19__670996-53-7-12
Average 92 stars, based on 15 article reviews
Endtag Nucleic Acid Labeling System, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/MB-9002/3'+EndTag+DNA+End+Labeling+System/bio_rxiv__2025__08__19__670996-53-7-12
Average 92 stars, based on 15 article reviews
endtag nucleic acid labeling system - by Bioz Stars,
2026-11
92/100 stars
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End Labeling:Article Title: Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Article Snippet: CRISPR–Cas interference is mediated by Cas effector nucleases that are either components of multisubunit complexes—in class 1 CRISPR–Cas systems—or domains of a single protein—in class 2 systems.. Here we show that the subtype III-E effector Cas7-11 is a single-protein effector in the class 1 CRISPR–Cas systems originating from the fusion of a putative Cas11 domain and multiple Cas7 subunits that are derived from subtype III-D. Cas7-11 from Desulfonema ishimotonii (DiCas7-11), when expressed in Escherichia coli, has substantial RNA interference effectivity against mRNAs and bacteriophages.. Similar to many class 2 effectors—and unique among class 1 systems— DiCas7-11 processes pre-CRISPR RNA into mature CRISPR RNA (crRNA) and cleaves RNA at positions defined by the target:spacer duplex, without detectable non-specific activity. Article Title: Structural Determinants of Arabidopsis thaliana Hyponastic Leaves 1 Function In Vivo Article Snippet: .. For fluorescence anisotropy titrations, RNA fragments were labeled with fluorescein using the Article Title: Protein-free ribosomal RNA scaffolds can assemble poly-lysine oligos from charged tRNA fragments Article Snippet: .. Labeling at the 5′-end was prepared with the Article Title: Protein-free ribosomal RNA scaffolds can assemble poly-lysine oligos from charged tRNA fragments Article Snippet: The 5′-biotinylated ptc1b RNA and ptc1a RNA molecules are purchased from IDTDNA. .. The 3′-ends are labeled with the Incubation:Article Title: Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Article Snippet: CRISPR–Cas interference is mediated by Cas effector nucleases that are either components of multisubunit complexes—in class 1 CRISPR–Cas systems—or domains of a single protein—in class 2 systems.. Here we show that the subtype III-E effector Cas7-11 is a single-protein effector in the class 1 CRISPR–Cas systems originating from the fusion of a putative Cas11 domain and multiple Cas7 subunits that are derived from subtype III-D. Cas7-11 from Desulfonema ishimotonii (DiCas7-11), when expressed in Escherichia coli, has substantial RNA interference effectivity against mRNAs and bacteriophages.. Similar to many class 2 effectors—and unique among class 1 systems— DiCas7-11 processes pre-CRISPR RNA into mature CRISPR RNA (crRNA) and cleaves RNA at positions defined by the target:spacer duplex, without detectable non-specific activity. Purification:Article Title: Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Article Snippet: CRISPR–Cas interference is mediated by Cas effector nucleases that are either components of multisubunit complexes—in class 1 CRISPR–Cas systems—or domains of a single protein—in class 2 systems.. Here we show that the subtype III-E effector Cas7-11 is a single-protein effector in the class 1 CRISPR–Cas systems originating from the fusion of a putative Cas11 domain and multiple Cas7 subunits that are derived from subtype III-D. Cas7-11 from Desulfonema ishimotonii (DiCas7-11), when expressed in Escherichia coli, has substantial RNA interference effectivity against mRNAs and bacteriophages.. Similar to many class 2 effectors—and unique among class 1 systems— DiCas7-11 processes pre-CRISPR RNA into mature CRISPR RNA (crRNA) and cleaves RNA at positions defined by the target:spacer duplex, without detectable non-specific activity. Fluorescence:Article Title: Structural Determinants of Arabidopsis thaliana Hyponastic Leaves 1 Function In Vivo Article Snippet: .. For fluorescence anisotropy titrations, RNA fragments were labeled with fluorescein using the Article Title: MYCN mRNA degradation and cancer suppression by a selective small-molecule inhibitor in MYCN-amplified neuroblastoma Article Snippet: .. For fluorescence titration, MYCN 3’UTR was synthesized by in vitro transcription with T7 polymerase (MAXIScript T7 RNA polymerase kit, Ambion), and labeled with fluorescence at its 5’end using the 5’ Labeling:Article Title: Structural Determinants of Arabidopsis thaliana Hyponastic Leaves 1 Function In Vivo Article Snippet: .. For fluorescence anisotropy titrations, RNA fragments were labeled with fluorescein using the Article Title: Protein-free ribosomal RNA scaffolds can assemble poly-lysine oligos from charged tRNA fragments Article Snippet: .. Labeling at the 5′-end was prepared with the Article Title: MYCN mRNA degradation and cancer suppression by a selective small-molecule inhibitor in MYCN-amplified neuroblastoma Article Snippet: .. For fluorescence titration, MYCN 3’UTR was synthesized by in vitro transcription with T7 polymerase (MAXIScript T7 RNA polymerase kit, Ambion), and labeled with fluorescence at its 5’end using the 5’ Article Title: Protein-free ribosomal RNA scaffolds can assemble poly-lysine oligos from charged tRNA fragments Article Snippet: The 5′-biotinylated ptc1b RNA and ptc1a RNA molecules are purchased from IDTDNA. .. The 3′-ends are labeled with the Article Title: Development of an RNA Aptamer as a Therapeutic Agent for Synucleinopathies Article Snippet: After washing, the membrane was probed with fluorescein isothiocyanate (FITC)-labeled aptamer (0.3 μM) or anti-αSyn115-121 antibody (clone LB509; BioLegend, San Diego, Japan) (0.5 μg/mL) at 25°C for 1 h. Chemiluminescent imaging was performed using LuminoGraph II (ATTO, Tokyo, Japan), with the VariRays II apparatus used exclusively for aptamer detection. .. RNA aptamers were biotinylated using the 5’ Plasmid Preparation:Article Title: Protein-free ribosomal RNA scaffolds can assemble poly-lysine oligos from charged tRNA fragments Article Snippet: .. Labeling at the 5′-end was prepared with the Article Title: Protein-free ribosomal RNA scaffolds can assemble poly-lysine oligos from charged tRNA fragments Article Snippet: The 5′-biotinylated ptc1b RNA and ptc1a RNA molecules are purchased from IDTDNA. .. The 3′-ends are labeled with the Titration:Article Title: MYCN mRNA degradation and cancer suppression by a selective small-molecule inhibitor in MYCN-amplified neuroblastoma Article Snippet: .. For fluorescence titration, MYCN 3’UTR was synthesized by in vitro transcription with T7 polymerase (MAXIScript T7 RNA polymerase kit, Ambion), and labeled with fluorescence at its 5’end using the 5’ Synthesized:Article Title: MYCN mRNA degradation and cancer suppression by a selective small-molecule inhibitor in MYCN-amplified neuroblastoma Article Snippet: .. For fluorescence titration, MYCN 3’UTR was synthesized by in vitro transcription with T7 polymerase (MAXIScript T7 RNA polymerase kit, Ambion), and labeled with fluorescence at its 5’end using the 5’ In Vitro:Article Title: MYCN mRNA degradation and cancer suppression by a selective small-molecule inhibitor in MYCN-amplified neuroblastoma Article Snippet: .. For fluorescence titration, MYCN 3’UTR was synthesized by in vitro transcription with T7 polymerase (MAXIScript T7 RNA polymerase kit, Ambion), and labeled with fluorescence at its 5’end using the 5’ other:Article Title: Systems, methods, and compositions for RNA-guided RNA-targeting CRISPR effectors Article Snippet: Gel extracted dsDNA products ( ̃1 μg/reaction) were used as input for the HiScribe T7 Quick High Yield RNA Synthesis kit (E2050S, New England Biolabs), with the 30 uL reaction size recommended for transcripts <300 nt. Article Title: smFRET study of rRNA dimerization at the peptidyl transfer center. Article Snippet: The PTC1a and PTC 1b are labelled at their 3′-end with kit from |